Immobilization of .BETA.-glucosidase in fibroin membrane.
نویسندگان
چکیده
منابع مشابه
Immobilization of Aspergillus beta-glucosidase on chitosan.
beta-Glucosidase of Aspergillus phoenicis QM 329 was immobilized on chitosan, using the bifunctional agent glutaraldehyde. The most active preparation based on the amount of support contained a 1:2.5 enzyme-to-chitosan ratio (wt/wt). However, the specific activity of the bound enzyme decreased from 10 to 1% with increasing enzyme-to-chitosan ratio. Compared with free beta-glucosidase, the immob...
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insulin has been suggested by Cuatrecasas & Illiano (197 1) on the basis of neuraminidase treatment of adipose-tissue cells. Enzymic digestion was reported to have no effect on the total quantity of insulin receptor in the membranes or on the affinity of the receptor for insulin, but abolished the effect of insulin on glucose transport and on lipolysis. As shown in Fig. 1, neuraminidase action ...
متن کاملScreening of Supports for the Immobilization of β-Glucosidase
A set of supports were screened for the immobilization of a partially purified extract of β-glucosidase from Aspergillus sp. These supports, namely, Eupergit, Amberlite, alginate, gelatin, polyvinyl alcohol- (PVA-) based matrices (Lentikats), and sol-gel, have proved effective for the implementation of some other enzyme-based processes. The initial criterion for selection of promising supports ...
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Acid beta-glucosidase (GCase) is a soluble lysosomal enzyme responsible for the hydrolysis of glucose from glucosylceramide and requires activation by the small nonenzymatic protein saposin C (sapC) to gain access to the membrane-embedded glycosphingolipid substrate. We have used in situ atomic force microscopy (AFM) with simultaneous confocal and epifluorescence microscopies to investigate the...
متن کاملMechanism of activation of glucocerebrosidase by co-beta-glucosidase (glucosidase activator protein).
The nature of the stimulatory action of the protein 'coglucosidase' on glucocerebrosidase was investigated with the use of highly purified cofactor from bovine spleen, radioactive glucosyl ceramide and methylumbelliferyl-beta-glucoside. A complex between glucosidase and either substrate could not be detected under equilibrium and non-equilibrium binding conditions. Complex formation between sti...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1978
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.42.1661